國立臺灣大學 農藝學系暨研究所

Hari Kumar Shrestha, Kae-Kang Hwu, Men-Chi Chang* (2010) Advances in detection of genetically engineered crops by multiplex polymerase chain reaction methods. Trends in Food Science & Technology 21: 442 - 454. (SCI)

 Men-Chi Chang*, Wei-Fu Chien, Chi-Hsein Chao, Mei-Kuang Lu * (2010) Effects of cold stress on alterations of physiochemical and chemical properties of rice polysaccharides. Carbohydrate Polymers 80: 374-377. (SCI)

Hari Kumar Shrestha, Kae-Kang Hwu, Men-Chi Chang* (2010) Detection of genetically modified maize (Zea mays L.) in seed samples from Nepal. African Journal of Biotechnology 9 (34): 5581-5589. (SCI)

1. 趙雲洋、劉麗飛、張孟基。2007。離層酸誘導之OsMAPK3水稻轉殖株可提升巴拉刈之抗性作物、環境與生物資訊 4:185-200
2. 趙雲洋、劉麗飛、張孟基。2007。抑制OsMAPK3基因表現會降低水稻對高鹽及乾旱的耐受性。臺灣農業化學與食品科學 45 (3): 142-154
3. 林維怡、劉麗飛、張孟基。2007。植物代謝體學在作物栽培和品質管控上之應用。科學農業55 (7, 8, 9): 1-11

1. Lin, S.K., M.C. Chang, Y.G. Tsai and H.S. Lur. 2005. Proteomic analysis of the expression of proteins related to rice quality during caryopsis development and the effect of high temperature on expression. Proteomics 5, 2140-2156.(SCI)
2. Chen, C.W., Y.W. Yang, H.S. Lur, Y.G. Tsai and M.C. Chang. 2006. A novel function of abscisic acid in regulation of rice (Oryza sativa L.) roots growth and development. Plant Cell and Physiology 47(1): 1-13.(SCI)
3. Chen Z. Y., M. C. Chang. 2004. Comparision of crop domestication, conventional crop breeding and genetic engineering. Scientific Agriculture 52(3,4):67-75
4. Chang M. C. 2002. The response of low-temperature stress and cold tolerance in plants. Chinese Agron. J. 12:131-139

1. Chang, M.C. 2005. Crops Physiological Response under Temperature Stress. Tea Survey and Protection Techniques. pp. 105-114. Published by Tea Research and Extension Station August, 2005.
2. Chen, C.C., I.C. Hung, M.C. Chang, S.J. Wang and L.F. Liu. 2005. Development of a quick method for detecting GM maize. Oral presentation. Annual Meeting of Chinese Society of Agronomy.
3. Chang M. C.,L.F Liu. 2004. The current status and perspectives of GM crops--increase the biosafety of GM crops by novel technology. Topic in crop modification: From debating to future
4. Lin S. K., M. C. Chang, Y. G, Tsai, H. S. Lur. 2004. Proteomic analysis of the expression of proteins related to rice quality during caryopsis development and the effect of high temperature on expression.3rd International Proteomics Conference (IPC’03) P-82.
5. La C. L., H. S. Lur, M. C. Chang . 2004. The analysis of physiological response and protein expression profile of different rice (Oryza sativa. L) cultivars under high temperature and stress.3rd International Proteomics Conference (IPC’03) P-76.
6. Yang Y. E., H. S. Lur, Y. G. Tsai, M. C. Chang . 2004. Proteomic analysis of differentially expressed proteins in roots of rice (Oryza sativa. L) seedlings under low temperature and ABA treatment.3rd International Proteomics Conference (IPC’03) P-22.
7. Lao C. L., H. S. Lur, M. C. Chang. 2004. The physiological response and protein expression profile of different rice (Oryza sativa L.) cultivars under high temperature stress. Chinese Agronomy society 2004 annual meeting. P.74
8. Chen C. W., M. C. Chang and H. S. Lur. 2003. Abscisic acid induces morphological and physiological changes in roots of rice (Oryza sativa L.) seedlings of cultivar Taichung Native 1. 2003 International Symposium on Plant Biotechnology.
9. Lin S. K., M. C. Chang, T. Y. Guang and H. S. Lur. 2003. Proteomic analysis of the expression of quality related proteins during rice caryopsis development and its response to high temperature. 2003 International Symposium on Plant Functional Genomics.A24
10. Chiang M. C., L. H. Chu, R. J. Tsai, H. Y. Liao, S. F. Lin and H. S. Lur. 2002. Comparison and Analysis of Granding Systems, Physical- Chemical Characteristics and DNA Fingerprints of Rice Grains from Local and Foreign Countries. Symposium of Grain Quality 81-99. The Chinese Agricultural Chemical Society.
11. Lin, S. K., Y. G. Tsay, M. C. Chang, and H.S. Lur. 2001.Proteomic study of grain development in rice: relationship between the expression and function of grain proteins and culture temperature.

Wei-Fu Jen, Chia-Ling Chang, Hsin-Hsiu Fang, Yun-Yang Chao, Mei-Kuang Lu, Li-Fei Liu, Tony H. H. Chen, Huu-Shen Lur, Men-Chi, Chang*Overexpression of OsMAPK3, At ICE and HvICE genes confers abiotic stress tolerance in rice (Invited speaker) Memorial Symposium for Professor Ray Wu (Oct 29, 2008)

1. 高庭姿、張孟基* (2009) 適用於去除環境中砷污染的復育植物—鱗蓋鳳尾厥(Pteris vittata.L). 科學農業 57 (1,2,3): 1-14.

2. 康家愷、張孟基* (2009) miRNA和siRNA之選殖鑑定、功能探討、及其在作物育種上的應用。科學農業 57 (4,5,6): 1-16.

3. 林維怡、劉麗飛、張孟基* (2007)植物代謝體體學在作物栽培和品質管控上之應用。科學農業55(7,8,9):1-11.

歷年指導學生研究情况

一、      大學部

89學年度: 鄭方逸     

90學年度: 莊汶博、謝亞雄

91學年度: 陳姿穎、張予馨(國科會大專生暑期研究計畫)、程永欽。

92學年度: 林祐瑩、鍾其芳 

93學年度: 簡芊卉、劉韻華

94學年度: 高紹芬、康家愷

95學年度: 高庭姿、高淑惠

96學年度: 簡維甫(國科會大專生暑期研究計畫) 、林依旋

97學年度:吳柏寬、洪偉勝、楊詠麟

98學年度: 楊祖豪、張靜華

99學年度: 許雅婷、陳筠婷、方廷方

二、      研究所

1. 88學年度-劉昌郎-水稻內外穎退化突變體相關農藝性狀及基因表現之研究(張孟基、劉麗飛)

2.89學年度-陳昭彣-水稻TN1幼苗根部在ABA誘導下之形態變化及生理研究 (張孟基、盧虎生)

3. .89學年度-楊雲瑋-低溫及離層酸處理下水稻幼苗根部之蛋白質體學分析

4. 89學年度-陳維平-水稻幼苗根部耐冷性相關基因之分離及鑑定

5. 90學年度-老嘉玲-高溫逆境下不同水稻品種之生理反應及蛋白質表現

6. 90學年度-趙雲洋(博)- 水稻OsMAPK3基因於非生物性逆境及除草劑耐性之功能研究(張孟基、劉麗飛)

7. 92學年度-陳姿穎-稻米礦物元素含量變異及產區判別之研究(張孟基、盧虎生)

8. 92學年度-李佳諭-以基因轉殖策略探討水稻OsATG8c

OsWRKY45之生理功能(張孟基、劉麗飛)

9. 93學年度- Hari K. Shrestha(博)- 基因改造玉米檢測方法之開發及及尼泊爾基改玉米現狀之監測 (張孟基、胡凱康、劉麗飛)

10. 94學年度-方信秀-過量表胞HvICE1或AtICE1(Inducer of CBF Expression)水稻轉殖株之分子鑑定及非生物逆境耐受性分析(張孟基、盧虎生)

11. 94學年度-陳柏宏-水稻OsAPK6/OsRK1基因之分子鑑定及功能性分析

12. 94學年度-劉秋萍-大量表現細胞週期相關基因AtCDC25、AtR2、及OsR2對菸草生長發育形態之影響(張孟基、劉麗飛)

13. 94學年度-林維怡-大量表現NTPHAN、SCR及WOX3基因於菸草對葉片形態之影響 (張孟基、劉麗飛)

14. 95學年度-黄雅貞(博)- 水稻肌動蛋白去聚合因子基因家族之功能性基因體分析(肆業中)

15. 95學年度-林盈甄-水稻心腹白相關基因OsWB之選擇性剪接基因表現與啟動子活性分析

16. 95學年度-王筱婷-水稻懸浮培養細胞於缺乏氮、磷、鉀逆境下之蛋白質體學分析(肆業中)

17.96學年度-張嘉玲-過量表現Mitogen-Activated Protein Kinase (OsMAPK3)水稻轉殖株之耐旱與耐鹽生理機制及基因表現分析

18. 97學年度-簡維甫-利用不同水稻品種探討糖類對水稻低溫逆境及耐受性之關係 (張孟基、盧美光)

19. 97學年度-楊雲瑋(博)- 比較三葉齡台農67號 (TNG67) 及台中在來1號 (TCN1)水稻幼苗於非生物逆境下，地上 (shoot) 及地下 (root) 部基因表現之差異(肆業中)

20. 97學年度-王泳傑-疊氮化鈉誘導台農67突變水稻及陸稻乾旱耐受性之生理機制探討(肆業中)

20. 98學年度-楊子儀-轉入嗜熱温泉菌DnaJ及DnaK基因之阿拉伯芥之非生物逆境耐受性測試(肆業中)

21. 99學年度-陳依甄- Genes, proteins expression pattern and functional characterization of bHLH (ICE-like) and ERF/AP2 (DREB) in rice(肆業中)

22. 99學年度-張靜華-國產水稻品種TNG67, TK6及KH145於不同生產階段下混米率之檢出

23. 100學年度-陳筠婷

24. 100學年度-蔡嘉倪

2007 重要研究成果

1. 完成國科會計畫”阿拉伯芥及大麥低溫耐性相關轉錄因子ICE及CBFs在轉殖水稻中之功能分析”。已產生單一基因考貝、同型結合且表現AtICE文HvICE及台農67號水稻轉殖株，對水稻之非生物逆境下耐受性具不同之影響。
2. 完成農委會計畫”白米單一核甘酸多型性分子標誌檢測技術之研發與實際應用”，本年度找出的SNP位點，TK2有14個、TK4有6個、TK8有44個、TK9有1個、TK14有14個、TK16有22個、KH139有25個、TN71有21個、越光有32個、M401有34個。此外在SNP genotyping之技術平臺開發方面，己發展4組ARMS (allele-specific; co-dominant) primer，並可對已有品種成功進行PCR genotyping。
3. 完成農委會計畫”水稻抗逆境相關突變株之分子鑑定及生理功能分析”。今年我們已完其中11株T-DNA突變株之Southern blot分析。此外我們以RT-PCR分析突變株中特定基因於非轉殖及突變株，不同生長發育時期、組織及之不同非生物逆境下表現量。目前正就農藝性狀及生理功能加以分析。